ABSTRACT
Aimand Objectives:Boerhavia diffusa(B. diffusa) frequently known as punarnava is specifically used to replenish the body. The present work was designed to evaluate the scavenging potentialof its bioactive constituents. Materials and Methods:The different fractions ofB. diffusaroot methanolic extractwere examined for phenolic, flavonoids contents, DPPH free radical and Nitric oxide scavenging activities. Further antioxidant activity wasevaluated by ABTS free radical scavenging method and also from the reducing potential scavenging activity. The total phenolic content in different fractions by using various solvent like ethanol, diethyl ether, chloroform, ethyl acetate, and n-butanol were carried out to find the antioxidant activities. Results:The phenolic content was highest in ethanolic fraction that was significantly comparable with ascorbic acid. The flavonoid content was highest in ethanol fraction (41.93 ± 3.92 μg/mL) followed by n-butanol fraction (31.68 ± 1.72 μg/mL), then ethyl acetate fraction (29.67 ± 2.83 μg/mL) and least in chloroform fraction (16.91 ± 2.74 μg/mL). The ethanolic fraction of B. diffusaalso showed highest DPPH radical scavenging activity (101.29 ± 3.78) when compared with other fractions of same extract using different solvent phases. Moreover the nitric oxide scavenging activity of ethanolic fraction was maximum (82.31 ± 2.83) than different fractions. The ethanolic fraction also showed improved ABTS radical scavenging activity (81.73 ± 2.73 mg/mL) while chloroform fraction showed poor ABTS radical cation scavenging activity (29.51 ± 2.79 mg/mL). Conclusions:The study concludes that Boerhavia diffusa has rich and ample source of phenolic acid and flavonoids. Among all fractions the ethanolic has potent antioxidant activity, which shows its significance for a better novel approach.
ABSTRACT
A rapid and feasible method of HPTLC is standardized for quantification of anethole in essential oil’s extract and from herbal formulations of fennel seed. The developeddensitometric HPTLC method was performed to estimate the existence of anethole in the essential oil, extract and herbal formulations of fennel with the optimized concentration of hexane: Ethyl acetate (8:2%, v/v, mobile phase) on glass coated silica gel 60 F254plates (20 × 10 cm) scanned with the absorbance of λ260nm under densitometric condition. The Linearity of regressions revealed a satisfactory relationship between peak area and concentration of anethole in between the range of 100-600 ng/spot. This reliable method was validated as per the ICH guidelines to fulfill the necessary parameters such as accuracy and robustness. The amount of anethole in essential oil (0.098 ± 0.002%),extract (0.101 ± 0.004%)and three herbal formulations A (0.024 ± 0.004%), C (0.019 ± 0.002%) whileanethole is not detected in B formulations from fennel seed was completely estimated by the developed method. The standardized methods and its validation gave new insights of HPTLC based detection and quantification of anethole in other aromatic plants as well as in other pharmacological formulations